Extended Data Fig. 8: Orthogonal slices of the Fourier Plane Correlation (FPC) of a ROSE-Z image of microtubule network.
From: Molecular-scale axial localization by repetitive optical selective exposure
a-c, Cross-sections of the qxqy-plane (a), the qxqz-plane (b), and the qyqz-plane (c) of the Fourier Plane Correlation, with resolution threshold contours indicating FPC = 1/7 (black lines). The correlation result indicated resolution of 23.0 nm, 29.8 nm and 20.4 nm in the X- Y- and Z- directions, respectively. Since the FPC show summed effect of localization precision, labeling error, feature of biological structure and system drift, so the resolution in axial direction did not show much advantage to lateral resolution in the results of FPC, and the resolution in X and Y also show greater difference due to the spreading direction of the microtubule network. d, Representation of the 3D microtubule network, with the axial coordinate in false color. Scale bar: 1 μm in d.
