Extended Data Fig. 1: One frame of a raw image from EMCCD in ROSE-Z.
From: Molecular-scale axial localization by repetitive optical selective exposure
Each frame of raw image with 512*512 pixels contains 4 subimages were indicated by subimage 1–4. Subimages 1–3 were used for the extraction of axial localization in ROSE-Z; subimage 4 was used for the demixing detection in multi-color imaging. The size of each subimage is approximately 220*220 pixels, with a pixel size of 150 nm, yielding a FOV of approximately 33*33 μm2. Scale bar: 10 μm. 5 experiments were repeated independently with similar results.
