Extended Data Fig. 4: Photos of the optical setup and the sample. | Nature Methods

Extended Data Fig. 4: Photos of the optical setup and the sample.

From: Molecular-scale axial localization by repetitive optical selective exposure

Extended Data Fig. 4

a, Close up of the chamber ready for STORM imaging, the top of the chamber was covered with another coverslip to create an optical window for illumination and was sealed with Vaseline. b, Close up of the microscope sample stage, showing the sample and the optics in the upper illumination path. The distance between the two coverslips was not restricted as in iPALM and 4Pi-SMS. c, The optical setup was built based on an Olympus IX71 inverted microscope.

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