Extended Data Fig. 6: Phase and phase difference across the FOV. | Nature Methods

Extended Data Fig. 6: Phase and phase difference across the FOV.

From: Molecular-scale axial localization by repetitive optical selective exposure

Extended Data Fig. 6

a, The scan positions of a fluorescent nanosphere which was used to calculate the phase and phase difference across the FOV. The scan was performed by a piezo stage within a region of 25 * 25 μm2. b, Distribution of three phases of the fluorescent nanosphere at each scanning positions within the FOV, with standard deviation of 7.2, 4.8 and 5.9 degrees, respectively. c, Distribution of the phase difference between phase1 and phase2 showing mean value of 119.9 degrees. d, Distribution of the phase difference between phase1 and phase3 showing mean value of -118.0 degrees (wrapped to a range of -180 to 180 degrees).

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