Extended Data Fig. 1: Overview of the DNA- and RNA-devoted library preparation of CoTECH. | Nature Methods

Extended Data Fig. 1: Overview of the DNA- and RNA-devoted library preparation of CoTECH.

From: Single-cell joint detection of chromatin occupancy and transcriptome enables higher-dimensional epigenomic reconstructions

Extended Data Fig. 1

a, Molecular structure of the CoTECH DNA-partition library. Following tagmentation and pre-amplification, the DNA-partition library was prepared by the 2-round PCR enrichment, resulting in the molecular constructs ready for sequencing using the standard Illumina Truseq recipe for paired-end 150-bp reads on Hiseq X-ten or Novaseq 6000 platform. b, Molecular structure of the CoTECH RNA-partition library. The mRNA was captured and reverse transcribed as described in a modified Smart-seq2 protocol. The reverse-transcribed cDNA was amplified by IS + 3’P2 primers and tagmented by Tn5-MEA. The resulting library was enriched by Nextera i5 and Truseq i7 primers and sequenced for paired-end 150-bp reads by loading both Nextera and Truseq read primers on the Novaseq 6000 platform.

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