Fig. 4: Stochasticity of single-cell contact maps reflects the intrinsic variability of single-molecule 3D conformations.
From: Comparison of the Hi-C, GAM and SPRITE methods using polymer models of chromatin
a, In silico single-cell distance maps (top) and average distance matrix (top right) of the Sox9 locus are shown. The variability of the corresponding single-molecule conformations of the model is represented in the bottom of the panel while the color scheme reflects the colors of the TADs of the locus19, shown in the color bar. b, The distribution of Spearman correlations between in silico single-cell distance maps and the average distance map is shown (mean value rs = 0.88). Mean Pearson and HiCRep correlations are reported in Supplementary Table 1c. c, In silico single-cell Hi-C, SPRITE and GAM contact maps corresponding to the first of the three in silico cells in a are shown (color scale indicates the percentiles of each map). Here, because the in silico efficiency is set to 1, all contacts are captured in Hi-C or SPRITE and all segregated windows are detected in GAM. d, The distribution is shown of Spearman correlation coefficients between in silico single-cell contact matrices at efficiency = 1 and their corresponding in silico single-cell distance matrices.
