Extended Data Fig. 2: LFM-SPIM optical setup.
From: Deep learning-enhanced light-field imaging with continuous validation
Schematic 2D drawing of the LFM-SPIM setup showing the main opto-mechanical components. The sample is illuminated through a single illumination objective with two excitation beam paths (ocra, light sheet illumination and blue, light field selective volume illumination) combined by a dichroic mirror (D1). The fluorescence is detected by an orthogonally oriented detection objective and optically separated onto two detection arms with a dichroic mirror (D2). Bandpass filters (BP1 and BP2) are placed in front of a tube lens (TL3,TL4) for the respective detection path. For the light field detection path (green), the tube lens (TL4) focuses on the microlens array (ML) and the image plane (shown in magenta) displaced by one microlens focal length is relayed by a 1-1 relay lens system (RL6) to an image plane coinciding with the camera sensor (shown in magenta). For the light sheet detection path, a combination of several relay lenses (RL1 to RL4), a 1:1 macro lens (RL5) together with a lens pair consisting of an offset lens (OL) and an electrically tunable lens (ETL) is used to image two axially displaced objective focal planes (shown in magenta, dotted and solid) to a common image plane at the sensor. The refocusing is achieved by applying different currents on the ETL. The mirror M1 is placed at a Fourier plane, such that the FOV of the light sheet path can be laterally aligned to fit the light field detection FOV. For single color imaging, the dichroic mirrors D1 and D2 are replaced by beamsplitters. See Methods for details.
