Extended Data Fig. 2: Quantification of 3D resolution. | Nature Methods

Extended Data Fig. 2: Quantification of 3D resolution.

From: Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution

Extended Data Fig. 2

Crimson fluorescent beads with 20 nm diameter were imaged in isoSTED mode. One-dimensional Lorentzian functions were fitted to images of isolated beads in both x and z directions. The full-width-half-maximum (FWHM) of the fitted functions are marked above each bead image. The numbers in green and red indicate the corresponding FWHMs in lateral (x) and axial (z) directions, respectively. All images are normalized to their peak intensities.

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