Extended Data Fig. 3: Effects of refractive index mismatch on the depletion and effective PSF. | Nature Methods

Extended Data Fig. 3: Effects of refractive index mismatch on the depletion and effective PSF.

From: Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution

Extended Data Fig. 3

The presence of refractive index mismatch between the silicone oil (ns) and the sample mounting medium (ni) causes the appearance of depth dependent side-lobes in the effective PSFs, even when correcting for piston. In addition, the main lobe of the effective PSF also shifts as a function of depth. All results are calculated for ns= 1.406 and ni = 1.33.

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