Extended Data Fig. 5: Sample holder design.
From: Three-dimensional adaptive optical nanoscopy for thick specimen imaging at sub-50-nm resolution
The specimens are mounted on a ring-shaped sample holder (a), between two coverslips at 5–10 μm (b, cells) or ~30 μm (c, tissue) distance. 100-nm gold beads are sparsely deposited onto the bottom coverslip for use during the aberration correction.
