Extended Data Fig. 6: Overcounting and undercounting in individual pixels.
Overcounting and undercounting in individual pixels: Comparison of four different thresholding methods with DDC in counting the number of true localizations in individual pixels on five spatial distributions as depicted and simulated in main text Fig. 2. The y axis is the difference between the true count and the method-identified count expressed as Count-[True Count], with positive values indicating the degree of over-counting and negative values the degree of under-counting. The x-axis is the number of true counts in individual pixels. The pixel size was set to 50 nm. Note that only DDC shows consistent distributions of y values near zero at different true count values and across all five spatial patterns. [Each scatter point is colored to illustrate the estimated probability density - allowing one to visualize the regions of high density (red) and regions of low density (blue).].
