Extended Data Fig. 9: Experimental Concerns. | Nature Methods

Extended Data Fig. 9: Experimental Concerns.

From: A pairwise distance distribution correction (DDC) algorithm to eliminate blinking-caused artifacts in SMLM

Extended Data Fig. 9

Image Error at different densities of localizations (a) and activation probability per frame (b). The raw data points are shown as gray points and the moving average is shown in black (Supporting Material). c, An intensity trajectory of a single mEos3.2 molecule with labels showing the definitions of Ton and Toff. d, The average Ton, Toff (per frame, frame rate 33Hz), and number of blinks for Alexa647 and mEos3.2 at different UV activation intensities (405 Power).

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