Fig. 3: Imaging data are already being reused. | Nature Methods

Fig. 3: Imaging data are already being reused.

From: REMBI: Recommended Metadata for Biological Images—enabling reuse of microscopy data in biology

Fig. 3

An example of a widely reused dataset is EMPIAR entry EMPIAR-10061 (https://empiar.org/10061), which contains the raw cryo-EM data (12.4 TB in size) underpinning what was a breakthrough structure and at the time the highest resolution cryo-EM structure available, the 2.2 Å resolution structure of β-galactosidase24. Several groups have reprocessed the data to even higher resolution and published and deposited the resulting EM maps. The dataset has been used by several developers of cryo-EM processing software to improve and test their algorithms, and it was used in the development of two deep-learning methods for automated particle picking, and to demonstrate cloud-based data processing. Details and literature references can be found at https://empiar.org/reuse.

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