Extended Data Fig. 1: Noise-controlled SIM reconstructions of GFP-zyxin protein in focal adhesions (green) and noise fraction map (magenta) over full FOV.
From: Structured illumination microscopy with noise-controlled image reconstructions
a–d, State-of-art SIM (w = 5 × 10−4), true-Wiener SIM, flat-noise SIM, and notch-filtered SIM reconstructions. Contours of the noise fraction map are added in white with contour level indicated. In all reconstructions the noise fraction is lowest in the foreground features and highest in the background region outside the cell. Overall, flat-noise SIM and true-Wiener SIM offer the lowest, and notch-filtered SIM the highest noise enhancement. Scale bar 5 μm.
