Extended Data Fig. 2: Multi-color noise-controlled 2D-SIM reconstructions.
From: Structured illumination microscopy with noise-controlled image reconstructions
a, Combined widefield, true-Wiener SIM, flat-noise SIM, and notch-filtered SIM reconstructions of a fluorescent test slide of a bovine pulmonary artery endothelial cell (red channel: mitochondria labeled with MitoTracker Red, green channel: actin labeled with Alexa Fluor 488, blue channel: DNA labeled with DAPI). Note that due to embedding in hardening mounting medium, cells are flattened and 3D nuclear morphology is compromised. b-f, Insets of the DAPI channel comparing state-of-the-art SIM with clear noise amplification artifact to the noise-controlled SIM reconstructions. The SSNR in the DAPI channel is low in this example case, due to reduced signal intensity and compromised morphology. The low SSNR is properly taken into account by the noise-controlled SIM reconstructions, without introducing artifacts, but not by the state-of-the-art SIM reconstruction. Scale bar (a) 10 μm, scale bar (b-f) 5 μm.
