Extended Data Fig. 3: Noise propagation in DMD-SIM.
From: Structured illumination microscopy with noise-controlled image reconstructions
a, Reconstructions of Alexa Fluor 488 labeled actin filaments in a bovine pulmonary artery endothelial cell with the iterative pattern-illuminated Fourier Ptychography (piFP) algorithm (see Supplementary Note 1) and with a band-pass regularization approach for flat-noise SIM. b, Comparison of flat-noise SIM to a widefield reconstruction obtained by summing the whole set of acquired images. c-e, Insets of the boxed region in (a) and (b). Both piFP and flat-noise SIM offer a resolution improvement, but piFP has better contrast than flat-noise SIM. The piFP reconstruction shows corrugated line structures and punctuated features (upper right of insets), similar to the structured noise artifact in state-of-the-art SIM with line illumination patterns, flat-noise SIM shows this to a lesser degree. Scale bar (a,b) 10 μm, scale bar (c-e) 4 μm.
