Extended Data Fig. 10: Expression patterns of site-specific glycan structures in different mouse tissues. | Nature Methods

Extended Data Fig. 10: Expression patterns of site-specific glycan structures in different mouse tissues.

From: StrucGP: de novo structural sequencing of site-specific N-glycan on glycoproteins using a modularization strategy

Extended Data Fig. 10

The 25 mass spectrometry raw files reported in the pGlyco 2.0 study were downloaded from the ProteomeXchange database. a, Overall processes of intact glycopeptide analysis of five different mouse tissues. b, Percentages of identified spectra that had at least 99% probabilities of related substructures from five mouse tissue data. Comparison of glycan core structures (c) and glycan subtypes (d) among intact glycopeptides identified by StrucGP from five mouse tissues. *indicates mouse brain glycopeptides identified from this study. e, Comparison of branch structures among five mouse tissues. The percentages in this figure were calculated based on the numbers of unique glycopeptides. Different isomers can be distinguished by specific feature B ions, the same method as shown in Extended Data Fig. 4.

Back to article page