Extended Data Fig. 6: Human donor PBMC QC analysis 3. | Nature Methods

Extended Data Fig. 6: Human donor PBMC QC analysis 3.

From: SCITO-seq: single-cell combinatorial indexed cytometry sequencing

Extended Data Fig. 6

a, UMAP (x-, y- axis with UMAP1 and UMAP2 dimensions) with representative PBMC markers based on CyTOF experiment using the same donor and antibody panel as in SCITO-seq. The scale shows arcsinh (hyperbolic inverse sine) transformed normalized values. b, Comparisons of SCITO-seq with CyTOF per donor (D1: top, D2: middle) for 100k loading data (SNG:singlet, DBL:doublet, TRI:triplet). Pairwise correlation heatmap plot (bottom) is also shown (similar to Fig. 2d and e in the main figure). Within donors, the proportion each Leiden cluster was highly correlated (Cosine similarity within donor1:0.95, donor2:0.94).

Back to article page