Extended Data Fig. 8: Scalability experiment and QC analysis 2 (60- and 165-plex).
From: SCITO-seq: single-cell combinatorial indexed cytometry sequencing
a, UMAPs of 60-plex (upper) and 165-plex (lower) experiment showing normalized expression of cDC1, cDC2 and pDC markers. CD141 and CD370 for cDC1 and CD1c for cDC2 and CD123 and CD303 for pDC markers. b, Schematic of sample multiplexed SCITO-seq where different samples are hashed with different pool barcodes (Red, Blue, Purple). Droplets containing cells from different individuals (two different colors) can be resolved into separate cells. c, Pairwise correlation plots (using the ggpair function from the GGally R package) of normalized expression values of all combinations for CD4 antibody in the 60-plex experiment. d, Pairwise correlation plots of normalized expression values of all combinations for CD4 antibody in the TSC 165-plex experiment.
