Extended Data Fig. 4: Human donor PBMC QC analysis (28plex).
From: SCITO-seq: single-cell combinatorial indexed cytometry sequencing
a, Ridgeplots of 28-plex experiment of the pool specific antibodies. The normalized expression values of 60 antibodies within each pool was summed for thresholding. An individual plot contains the batch specific normalized expression values for demonstrating the signal to noise distribution of the expected specificity. b, UMAP projections for 100k PBMC loading experiment for representative markers. UMAP projection comparison using RNA expression (left), merged antibody counts before resolution (middle) and after resolution (right). The scale represents the normalized merged ADT counts (left and the middle) and resolved ADT counts (right).
