Extended Data Fig. 5: Human donor PBMC QC analysis 2.
From: SCITO-seq: single-cell combinatorial indexed cytometry sequencing
a, RNA expression based UMAP projections for representative markers of 200k PBMC loading. Since the RNA molecules are not combinatorially indexed, these UMAPs show stark contrast with the resolved UMAP based on normalized ADT counts where we see clear distinction of all clusters. b, UMAP ADT projection of 200k loading PBMC data colored by different pools (color numbers 0 through 9). Two pooled donors prior to aliquoting into 10 different pools to investigate batch effects across all stained wells and found no apparent batch effects. c, ADT UMAP clusters overlaid on ADT UMAP (left) and ADT UMAP clusters overlaid on transcriptomic UMAP. d, (top) Protein expression on ADT UMAP of CD4/8 and CD45RA/RO. (down) Protein expression on transcriptomic UMAP of CD4/8 and CD45RA/RO.
