Extended Data Fig. 10: Cross-system validation. | Nature Methods

Extended Data Fig. 10: Cross-system validation.

From: Reinforcing neuron extraction and spike inference in calcium imaging using deep self-supervised denoising

Extended Data Fig. 10

Denoising performance of DeepCAD on three two-photon laser-scanning microscopes (2PLSMs) with different system setups. Our system was equipped with alkali PMTs (PMT1001, Thorlabs) and a 25×/1.05 NA commercial objective (XLPLN25XWMP2, Olympus). The standard 2PLSM was equipped with a GaAsP PMT (H10770PA-40, Hamamatsu) and a 25×/1.05 NA commercial objective (XLPLN25XWMP2, Olympus). The two-photon mesoscope was equipped with a GaAsP PMT (H11706-40, Hamamatsu) and a 2.3×/0.6 NA custom objective. The same pre-trained model was used for processing these data. All scale bars represent 100 μm.

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