Extended Data Fig. 9: Bleaching levels for different excitation powers and estimated focal energies of imaging conditions utilized in-vivo using Thy1-GFP mouse at 630 μm depth.
a, Fluorescence signal level versus number of imaged frames for different surface power levels. The corresponding estimated focal energies were estimated based on attenuation length (EAL = 275μm, Extended Data Fig. 2). b, Images acquired with (Left) 3.55 mW and (Right) 2.1 mW surface power, as utilized during typical high-resolution imaging and AO measurements, respectively, corresponding to yellow and blue bleaching curves in (a). From (a) both imaging conditions show negligible bleaching effects and are below <1nJ pulse energy, thus well within the previously established safe pulse energy regime7. Scale bar 20μm. Representative result of (n=3) experiments.
