Extended Data Fig. 1: Schematic experimental set-up. | Nature Methods

Extended Data Fig. 1: Schematic experimental set-up.

From: High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

Extended Data Fig. 1

Femto-second laser pulses at 1300 nm are generated by a NOPA system pumped by a fiber-based amplified laser. Laser pulse duration is maintained at the sample plane with a custom-build single-prism pulse compressor. The deformable mirror, X and Y galvo mirrors, and the wavefront sensor are conjugated to the objective pupil plane with 4-f relay lens pairs. Fluorescence signals are collected by large-aperture optics and detected by large-area PMTs. Physiological parameters of the mouse (ECG, breath rate, etc.) are processed in real-time by an FPGA which in turn gates the image acquisition of the microscope.

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