Extended Data Fig. 4: Image quality improvement for in-vivo imaging with aberration correction in Hippocampus.
Images were recorded in Thy1-EGFP(M) mice with two different conditions. AO system: wavefront correction of system aberrations; AO full: wavefront correction of system and brain tissue aberrations including Z35 modes (excluding piston, tip and tilt) for correction. Additionally, cardiac gated image acquisition was performed for both AO system and AO full condition. a, Maximum intensity projection images of orthogonal view (xz) for the two different conditions at 877-1193μm depth. AO measurement was performed on somata indicated by white box. After aberration correction and intensity enhancement was achieved over the entire FOV, illustrated by intensity line plots across somata (b) and dendrites (c) indicated in (a) by straight and dotted white line, respectively. d, Maximum intensity projection images (top) and orthogonal view (bottom) for system and full AO correction at 970-1020μm depth. (e) and (f) display lateral intensity plot along dendrites and somata, respectively, indicated in (d) by white dotted and white straight line, respectively. h, Spectral power map as a function of spatial frequency (wavenumber) and (g) average radial profile of spectral power maps for maximum intensity images in (d). Scale bar 20μm. Representative result of (n=3) and (n=8) experiments in (a-b), respectively.
