Extended Data Fig. 5: Characterization of initial SBR condition on AO measurement performance in-vivo at ~600μm in Thy1-GFP mouse at 1300 nm excitation.
a, Initial image quality of neuron somata used for AO measurement under three different SBR conditions calculated on raw images (Right). For calculation of the image quality metric the raw images were (Left) median filtered (7x7pixel kernel) and thresholded so that only the somata signal (indicated by white dotted circle) was used for analysis. b, Intensity ratio (AO on/AO off) versus iteration number for the three different initial SBR conditions.
