Extended Data Fig. 8: Effect of GTP and GMPPNP on the oligomeric distribution of WT. | Nature Methods

Extended Data Fig. 8: Effect of GTP and GMPPNP on the oligomeric distribution of WT.

From: Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers

Extended Data Fig. 8

Average oligomeric distribution of 20 nM WT in contact with an SLB in its apo-state, with 1 mM GTP, with 1 mM of GMPNP (non-hydrolysable GTP analogue) and with 1 mM MgCl2 instead of 2 mM MgCl2. The 1 mM MgCl2 measurement was included as a control in case the presence of GTP/GMPNP results in a reduction in free Mg2 + , which could potentially affect the membrane affinity of WT dynamin oligomers. Each bar plot was generated by taking the mean oligomeric counts from n = 3 repeat measurements (1 min dynamic MP movie each). Error bars are presented as mean values ± s.d..

Source data

Back to article page