Extended Data Fig. 10: Effect of the window size of the sliding median filter on particle contrast.
(a) Mean contrast of WT dimer (red circles), tetramer (orange squares), hexamer (purple crosses) and octamer (blue diamonds) trajectories, (b) mean contrast vs mass calibration slope obtained from the dynamic MP movie in (a) vs single frame length after averaging and (c) contrast precision of our PSF-fitting procedure for each oligomer (same symbols as in (a)) all plotted vs total exposure time of 1 frame after averaging. The plots were obtained from the same movie of WT used in Figs. 1b-d and 2a (n = 1 movie (4 min) of 20 nM WT) with additional frame averaging of 1, 2, 3, 4 and 5 frames, which corresponds to frame lengths of 3.02, 6.04, 9.05, 12.07 and 15.09 ms or frame rates of 331, 166, 110, 83 and 66 Hz, respectively (see Supplementary Information). The data in (a) and (c) are presented as mean values ± s.e.m. for each oligomeric species. The data in (b) is presented as mean values ± s.d.. For these plots ndimer = 34, 51, 60, 52, 73, Ntetramer = 82, 102, 98, 97, 94; nhexamer = 177, 229, 224, 208, 173; noctamer = 22, 29, 37, 38, 33 trajectories for total exposure times of 3.0, 6.0, 9.1, 12.1, 15.1 ms, respectively.
