Extended Data Fig. 2: Target Genes in Response to Cytokine Treatments.
From: Systematic investigation of cytokine signaling activity at the tissue and single-cell levels
a, Differential expression of target cytokines (x-axis) regulated by IL4 or BMP6 (y-axis). The thick line represents the median value. The bottom and top of the boxes are the 25th and 75th percentiles (interquartile range). The whiskers encompass 1.5 times the interquartile range. The difference between group values and zero among treatment profiles that passed quality controls was tested using the two-sided Wilcoxon signed-rank test, with p-values and sample counts labeled. b, Gene set enrichment among cytokine response targets. The normalized enrichment score from the gene set enrichment analysis (GSEA)6 represents the overall enrichment of pathway members among target genes from each cytokine’s composite signature. c, Normalized enrichment scores from panel b among pairs of hallmark categories and cytokines. d, Gating strategy of flow analysis. The forward scatter height (FSC-H) and side scatter height (SSC-H) are used for dead cell and cell debris removal. FSC-H width and SSC-H width are used to gate the single cells. We include unstained cells to define the gate threshold that separates positive populations and negative control cells.
