Extended Data Fig. 4: RNA modifications detected using LC-MS/MS, dot-blot assays and MeRIP-seq/m6A-seq.
From: Systematic calibration of epitranscriptomic maps using a synthetic modification-free RNA library
a) LC-MS/MS detection for m6A, m1A and m5C in mESC cellular mRNA, IVT RNA and mRNA from two Mettl3 KO cell lines. b) Dot-blot assays of m6A for mESC cellular mRNA and IVT RNA. c) LC-MS/MS and dot-blot assay of m6A for the modification detection in FTO-treated mRNA. d) IVT RNA or Mettl3 KO samples are applied as negative controls for m6A peak calling. The peak summits are determined to represent the peak position for plotting.
