Extended Data Fig. 8: Quantitative presentation of the impact of heterogeneity in culture medium on size of spheroids.
From: MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
Left, Z-score heatmaps and right, violin plots presenting the impact of six different media types on spheroid size difference in (a) 8 established cell lines and (b) 3 early passage, patient-derived sarcoma cultures. Biological replicates are indicated by a different symbol (N ≥ 3); each symbol is a technical replicate (n = 8). Y-axis represents log2-transformed changes in size, all media types are normalized to DMEM HG. Horizontal bar indicates median. Statistical significance between the groups was determined with a one-way ANOVA and Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001, ****p < 0.0001. Colors in violin plots present media type as in Fig. 3a; media types are ranked from higher nutrient (left) to lower nutrient (right) richness.
