Extended Data Fig. 9: Response to radiotherapy of spheroids cultured in diverse media types.
From: MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
Bar plots (a and c); and Z-score heatmaps (b and d) respectively indicate the impact of a single (a and b) 20 Gy and (c and d) 10 Gy fraction on ATP content of spheroids of indicated cell lines cultured in different media types. Biological replicates are indicated by a different symbol (N = 2; except HEPG2 N = 1); each symbol is a technical replicate (n = 8). Statistical significance between the groups was determined with a one-way ANOVA and Tukey’s multiple comparison test. **p < 0.01, ***p < 0.001, ****p < 0.0001. Horizontal bar indicates median, error bars indicate SD. Colors in bar plots present media type as in Fig. 3a; media types are ranked from higher nutrient (left) to lower nutrient (right) richness.
