Extended Data Fig. 10: Evaluation of the metrics cell death, circularity and size in an interlaboratory study.
From: MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
(a) Correlation matrix of cell death ranking of HCT116 spheroids cultured in six different media types between different participating sites in the interlaboratory study (left) and between biological replicates in the study-initiating laboratory (right). In (b) and (c), Z-score heatmaps (left) and correlation matrices (right) present the impact of spheroid circularity (b) and size (c) of HCT116 spheroids cultured in six different media types evaluated across seven different sites in an interlaboratory setting. Media types in Z-score heatmaps are ranked from higher nutrient (left) to lower nutrient (right) richness. The median Spearman correlation across the entire dataset is respectively 0.56 for circularity and 0.57 for size. The larger spread in correlation coefficient of circularity across laboratories is due to subtle absolute differences with most probably limited biological significance.
