Fig. 4: Culture media-induced heterogeneity in spheroid metrics across multiple cell types.
From: MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity
a, Spider plots of metrics from spheroids of indicated cell lines cultured in six different medium types. Axes represent the Z-score metrics of cell death, ATP content, L/G ratio, secreted protein signatures of angiogenesis and immune interaction, circularity, size and therapy response. A higher Z-score means a higher metric value. The left and middle columns indicate established cell lines; early passage and patient-derived sarcoma cultures are on the right. b, Cell death of HCT116 spheroids cultured in six different medium types evaluated at seven different laboratories (“sites”) in an interlaboratory experiment. At each site a higher ranking indicates a higher cell death. Each dot represents an evaluated spheroid. The colors indicate the medium type as in a.
