Fig. 4: Analysis and verification of the ‘Hex+17 (aH)’.
From: Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3
a, Unlabeled aH-N-glycopeptides are identified on fission yeast data (PXD005565) and verified by the 15N-/13C-labeled MS1 precursors and the N-glycome MS/MS data. Unlabeled aH-O-Man glycopeptides are also identified and verified by 15N-/13C-labeled MS1 evidence. b, MS/MS spectral annotation (top) and 15N and 13C MS1 evidence (bottom). In the MS1 annotation, R is the Pearson correlation coefficient between the theoretical and experimental isotope distributions. The blue square and green circle refer to HexNAc and Hex, respectively. The MS2 spectrum is a chimera spectrum of ‘VQASJ(N)WTGTR + Hex(9)HexNAc(12)aH(1)’ and 15N-labeled ‘VQASJ(N)WTGTR + Hex(10)HexNAc(2)’ (Supplementary Data). c, Deep analysis of aH-N-glycopeptides and aH-O-Man glycopeptides in fission yeast and budding yeast data. Phospho-Hex (phoH) glycopeptides are also found in budding yeast samples; 262 N-glycopeptides and seven O-Man glycopeptides in budding yeast contain both aH and phoH.
