Extended Data Fig. 3: Team-centric overview of the search output data from the glycopeptide identification process (SO1-SO9). | Nature Methods

Extended Data Fig. 3: Team-centric overview of the search output data from the glycopeptide identification process (SO1-SO9).

From: Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis

Extended Data Fig. 3

Distribution of the a. LC retention time (min), ***P = 4.52 ×10-6, b. observed glycopeptide m/z, c. observed charge state (z), *P = 1.97 ×10-2, d. observed precursor selection off-by-X (Da, positive values only), e. observed glycopeptide mass [M + H]+ (Da), f. actual mass error of observed glycopeptides (ppm, positive values only), **P = 2.78 ×10-3, g. length of observed glycopeptides, ***P = 2.44 ×10-6, h. glycan mass of observed glycopeptides (M, Da), ***P = 1.03 ×10-9, i. total N- and O-glycoPSMs reported by the participants, ***P = 1.02 ×10-4. The mean and SDs of data from all teams are also indicated for each graph. Developer data are plotted in orange and user data points are in blue. Teams reporting data outside the SDs have been labelled. The N-glycopeptide (N-GP, n = 22) data were statistically compared to the O-glycopeptide (O-GP, n = 20) data using unpaired two-sided t-tests where *P < 0.05, **P < 0.01 and ***P < 0.001. See Supplementary Table 4 for data.

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