Extended Data Fig. 1: Biotin-DAP-seq in Arabidopsis thaliana.
From: Persistence and plasticity in bacterial gene regulation
Demonstration of successful biotin-DAP-seq with eukaryotic transcription factors amplified directly from cDNA. The Arabidopsis thaliana TFs AT3G12730, AT1G72010, and AT1G77920 were selected to represent three distinct TF protein families: MYB, TCP, and bZIP, respectively. The identified binding signal obtained using biotin-tagged proteins matches closely to DAP-seq with Halo-tagged proteins as used in the original 2016 DAP-seq publication.
