Extended Data Fig. 2: Fragment size in DAP-seq is a tunable parameter.
From: Persistence and plasticity in bacterial gene regulation
DAP-seq fragment library insert size is a tunable parameter, depending on the desired resolution. For multiDAP experiments presented in this work, libraries were constructed from genomic DNA sheared to an average size of 75 bp, because we found this to offer high resolution while still accurately capturing known binding sites. Note that at extremely short insert sizes (20 bp, bottom track) the signal begins to decay, likely because the size is too short to capture the local DNA context of clustered AgaR motifs.
