Extended Data Fig. 3: The multiDAP assay produces unique and reproducible TF binding maps.
From: Persistence and plasticity in bacterial gene regulation
MultiDAP with 92 E. coli TFs along with 4 negative control samples, run in three independent triplicate 96-well plate experiments demonstrates unique binding signatures for each TF, which are highly reproducible. Signal correlation among these 288 samples was assessed by splitting the entire E. coli genome into 25 bp bins, each with an assigned signal value determined by the sequence read pileup within that bin. Inset shows detail with strong agreement between triplicates, while negative control wells with mock TF expression are largely un-correlated. This implies that background signal is primarily random noise, while individual TFs specifically enrich for binding site regions. Median correlation between replicates is 94%. See also Supplementary Table 4 for peak numbers and correlations.
