Fig. 3: Scaling light microscopy metadata guidelines with experimental, technical and analytical intent and complexity minimize recordkeeping burden while maximizing value, quality and reproducibility of image data. | Nature Methods

Fig. 3: Scaling light microscopy metadata guidelines with experimental, technical and analytical intent and complexity minimize recordkeeping burden while maximizing value, quality and reproducibility of image data.

From: Towards community-driven metadata standards for light microscopy: tiered specifications extending the OME model

Fig. 3

Shown is a schematic representation of the graded system for metadata specifications proposed by 4DN and BINA to tailor reporting requirements to experimental complexity. In this system, microscope hardware and imaging experiments are classified based on the following criteria: (1) experiment and image complexity, (2) microscope technology and imaging modality and (3) results and analysis requirements. For each criterion, the schema provides graphical illustrations of increasing complexity along the three-tier axis that can be used as an initial guide for microscope users in mapping reporting requirements to their experimental needs.

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