Table 3 Examples of utilization of the three-axis matrix of the 4DN-BINA-OME Microscopy Metadata Specifications
No. | Description | Sample | Res. | Image structure | Analysis | 1st axis: tier | 2nd axis: OME extension | 3rd axis: requirement level |
|---|---|---|---|---|---|---|---|---|
1 | Investigation of PTC299 effect on SARS-CoV2 replication in Vero cells using wide-field commercial microscope | Fixed cells | Low | Dual- channel; multi-point | Simple segmentation and intensity quantification | 1 | C + B | Required only |
2 | High-resolution OligoPaint investigation of chromatin structure using commercial wide-field microscope | Fixed cells | High | Multi- channel; 3D z-stack | Spot detection and subpixel localization | 2 | C + B + CP | Required only |
3 | High-resolution, real-time tracking of export of HIV-1 genomic RNA from infected T cells using custom made microscope | Live cells | Super | Multi- channel; 3D z-stack; time-series | Spot detection, subpixel localization and tracking | 3 | ALL | ALL |
4 | Wide-field microscope manufacturers | Any | Any | Any | Any | 3 | C + B + CP | ALL |
