Extended Data Fig. 8: Spontaneous Ca2+ activity in the awake versus anesthetized state.
From: Fast optical recording of neuronal activity by three-dimensional custom-access serial holography
(a) 3D coordinates of cells in the dataset (47 cells). (b) GCaMP6f Fluorescence images of all cells. (c) Fluorescence traces in false colors, according to the color bar on the right side of (e) recorded in the awake state. Color bar on the top indicates locomotion velocity of the animal. (d) Spikes found in (c) by MLspike. (e) Fluorescence traces from the same cells as in (c), but with the mouse under 0.25 % isoflurane anesthesia. (f) Spikes found in (e) by MLspike. (g) Histogram of pairwise spike correlations in (d) between all cells (black), between layer 2/3 cells (red) and between layer 5 cells (blue). Spikes were counted as synchronous if they fall within a 10 ms time window. (h) Same as in (g), but evaluated from the dataset in (f). (i) Mean spike rates calculated from the spike maps in (d) and (f). Paired t-test, p = 2·10−4 (***; 47 cells), 2·10−3 (**; 36 cells), 1·10−2 (*; 10 cells). Pairs of same cells connected by lines (gray).
