Extended Data Fig. 3: Event detection accuracy evaluated on simulated data.
From: Fast optical recording of neuronal activity by three-dimensional custom-access serial holography
a) Workflow schematic: We generated GCaMP6f-like neural calcium activity (racq = 250 Hz, 600 spikes, mean firing rate 0.2 Hz, 3000 s) contaminated with a neuropil signal. After neuropil correction, we deconvoluted the data and from inferred spikes evaluated the error rate (ER), the sensitivity and the precision of inference. (b) Error rates for artificial data (SNR 2, 3 and 4) according to neuropil correction (noise- or correlation regression) and inference algorithm (MLspike and BSD). (c) Error rate as function of acquisition rate, racq, for data with SNR between two and five obtained with the BSD algorithm for two window sizes ΔTwin (12 and 20 ms). A spike is rated as detected, if the spike falls within this time window centered around the time of the true spike.
