Extended Data Fig. 6: Tracking spatial distribution of nuclei lineages using Light sheet microscopy.

(a) Example images show a nucleus dividing into two daughter nuclei in the developing organoid. White circles represent manual detection and tracking over time. (b) Scatter plot shows increase in the number of nuclei over 100 hours of tracking in lineage one (L1). The curve shows the exponential model estimated by the data, with the estimated doubling time being 17.3 hours. (c) Nuclei tracking of three lineages (L1-L3) in the same lumen area (top) and of a fourth lineage (L4) surrounding a diametrically opposite lumen (bottom). Scale bar is 100 µm in all images. (d) Lineage trees for the four tracked nuclei lineages. (e) Spatial distribution in the x-y plane of the four tracked lineages. Nuclei are shown at 0 hours and 65 hours, colored by lineage. (f) Dotplot shows the increase in the number of daughter nuclei for all four lineages over 65 hours.