Extended Data Fig. 1: Progress of a typical enzymatic reaction.
From: Monitoring protein conformational changes using fluorescent nanoantennas
(a) The states of an enzyme typically present during the catalysis of a substrate: the enzyme and substrate(s) (E + S) are introduced, followed by formation of the enzyme-substrate complex intermediate (ES). Next is the transition state (ES‡), followed by the enzyme-product complex intermediate (EP), and finally the enzyme and released product(s) (E + P). (b) Representative changes with time in concentration of the substrate ([S]), product ([P]), enzyme ([E]) and enzyme-substrate complex ([ES]) are shown. See Supplementary Note 1 for further discussion of important studies of methods to detect the different states of AP.
