Extended Data Fig. 4: NTR 2.0 does not enhance ablation efficacy with the prodrug nifurpirinol.
a, Transgenic zebrafish larvae co-expressing YFP and NTR 2.0 in neurons (NRSE:KalTA4;UAS:YFP-NTR2.0) were exposed to the indicated concentrations of NFP for 24 h (5- 6 dpf) and YFP levels were quantified by plate reader at 7 dpf (n = 3 biologically independent experiments, dot plots show the number of larvae examined). Box plots show first quartiles (25th percentile), medians, third quartiles (75th percentile), and whiskers = SD, with individual data points (larvae) overlaid as a dot plot. Fully detailed statistical comparisons (absolute effect sizes, 95% confidence intervals, Bonferroni-corrected p′-values derived from two-tailed t tests, and sample sizes) between NFP-treated and control conditions in graphs b and d are provided in Supplementary Table 5.Symbols: #p′ > 0.05, *p′ ≤ 0.05, ****p′ ≤ 0.0001; ○ = outlier data points.
