Extended Data Fig. 5: Comparative photobleaching and phototoxicity rates of N-SIM and IDDR-SPIM. | Nature Methods

Extended Data Fig. 5: Comparative photobleaching and phototoxicity rates of N-SIM and IDDR-SPIM.

From: Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales

Extended Data Fig. 5

Time-lapse images of ER in live U2OS cells by 2D N-SIM and IDDR-SPIM are shown in a and b, respectively, indicating notably lower photobleaching and phototoxicity by IDDR-SPIM. 9 raw frames were acquired to reconstruct a single N-SIM image. To yield similar SNR in the reconstructed images at the initial time point, the illumination intensities for 2D N-SIM and IDDR-SPIM were 50 W/cm2 and 1 W/cm2, respectively.

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