Extended Data Fig. 9: Extending the imaging depth through the integration of axial scanning with DR-SPIM. | Nature Methods

Extended Data Fig. 9: Extending the imaging depth through the integration of axial scanning with DR-SPIM.

From: Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales

Extended Data Fig. 9

a, An entire expanded nuclear of U2OS cell (~4×) imaged via axial scanning-enabled DR-SPIM. Scale bar: 10 μm. Combined with the axial scanning scheme, the imaging depth of DR-SPIM could be increased from ~10 μm to ~50 μm while keeping the same high axial resolution. b, X-y, x-z, and y-z plane MIPs of the boxed region, showing the fine structures of nuclear pores. Scale bar: 1 μm.

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