Extended Data Fig. 7: Comparison of the efficiencies of accurate 150 bp insertion using GRAND editing and PE3 at five endogenous loci.
From: Efficient targeted insertion of large DNA fragments without DNA donors
a, Detection of the accurate 150 bp insertion at five sites edited by GRAND or PE3. The target regions were amplified and the PCR products were digested by HindIII restriction enzyme. The digested products were displayed by 2% TAE agarose. The digested products were indicated by the red arrows. The predicted sizes of digestion products with precise editing are listed below the image of agarose gel. b, Detecting the accurate 150 bp insertion and imperfect events of GRAND or PE3 by deep sequencing. Mean ± s.d. of n = 3 independent biological replicates.
