Extended Data Fig. 8: Comparison of the insertion frequencies of large DNA fragments with different lengths of complementary base pairs.
From: Efficient targeted insertion of large DNA fragments without DNA donors
a, Insertion of 458 or 600 bp DNA fragments at EGFP site with 60 or 200 bp complementary base pairs. b, Insertion of 767 or 1085 bp DNA fragments at EGFP site with 50 or 200 bp complementary base pairs. dGRAND: dead Cas9-RT + dual pegRNAs, used as control for deep sequencing analysis; GRAND: nick Cas9-RT + dual pegRNAs. Mean ± s.d. of n = 3 independent biological replicates.
